FIG. 1.
Macrophage depletion after subcutaneous FP injection of CCL. Seven days post injection, cells from the spleen, pLN and ingLN were analyzed for the expression of CD11b, CD11c, F4/80 and MOMA-I (CD169) by flow cytometry. (A) Schematic showing lymphatic drainage from the footpad. The percentage of (B) CD169+ CD11c− cells (SCS macrophages), (C) CD11b+ F4/80+ cells (medullary macrophages) or CD11b+, CD11c+ (dendritic cells) from CCL- or PBSL-treated mice are shown. Error bars indicate standard deviation (3 mice per group). P value determined by Student’s t test.