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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Virology. 2014 Jan 10;0:278–289. doi: 10.1016/j.virol.2013.12.021

FIG. 8.

FIG. 8

Cytokine production by WNV-specific CD8+ T lymphocytes is not affected by depletion of SCS macrophages. CCL- or PBSL treated B6 mice (n=6) were inoculated in the FP with 107 IU RepliVAX WN. Lymphocytes from spleen and pLN were harvested on day 5 (n=2) or day 7 pi (n=4), stimulated and assayed for production of cytokines as described in methods. (A) Representative staining for IFN-γ and TNF-α in splenocytes stimulated with NS4B peptide, irrelevant peptide, or medium. The mean (± SD) frequency (B, D, F, H) or total cell number (C, E, G, I) of cytokine producing CD8+ T cells from the spleen or pLN are shown (*p <0.05).