Fig. 3.

Resveratrol and AMPK activator effects during polyploidization. (A and B) p53-deficient human colon carcinoma HCT116 cells (A) and p53^−/− mouse mammary gland epithelial cells (MMECs) (B) were left untreated (control) or incubated for 48 h with the indicated drugs in combination or not with the polyploidizing agents nocodazole 100 nM (A) or dihydrocytochalasin B 1 μM (B). The following concentrations were used (for HCT116 and MMECs, respectively, if different): resveratrol (40 μM), rolipram (0.8 and 1 mM), A-769662 (0.4 and 0.2 mM), aspirin (10 mM), salicylate (10 mM), 2-deoxyglucose (40 and 1 mM), and 3-bromopyruvate (80 μM). At the end of the incubation, DNA content was assessed by cytofluorometry upon Hoechst 33342 staining. Representative cell cycle profiles (Upper) and quantification of polyploid (>4n) cells (Lower) are reported. (Upper) Numbers refer to percentage of polyploid cells. The results are reported as mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001 (Student t test), compared with the cells exposed to nocodazole or dihydrocytochalasin B alone (Lower). See also Fig. S9.