Fig. 4.

Cell fate profiling of newly generated polyploid cells treated with resveratrol or salicylate. (A and B) p53-deficient human colon carcinoma HCT116 cells stably transfected with H2B-GFP were exposed to the indicated drugs combined or not with nocodazole to induce polyploidization. Cell fate was monitored by videomicroscopy for 72 h. Representative pictures of cells treated with nocodazole alone or in combination with resveratrol are shown in A (arrowheads indicate relevant events). In B, the cell fate profiles of 50 cells left untreated (control) or incubated with resveratrol or salicylate, in combination or not with nocodazole are reported. Light gray depicts interphase cell; orange, successful division; yellow, abortive mitosis; red, interphase apoptosis; and purple, mitotic apoptosis. Gray darkening depicts a change in nuclear size and ploidy, from diploidy to decahexaploidy.