Fig. 3.

The role of ADPR in activation of Ca²⁺ entry in hepatocytes. (A) Poly-ADPR–specific immunofluorescence in rat control hepatocytes (A, i) and hepatocytes treated with 10 mM acetaminophen (45 min in A, ii; 16 h in A, iv) or 1 mM H₂O₂ (45 min in A, iii). (B and C) Inhibition of H₂O₂ and acetaminophen-induced Ca²⁺ entry in hepatocytes by PARP inhibitor DPQ (10 μM). DPQ was added to the incubation medium 2 min before the addition of H₂O₂ or acetaminophen (n = 3).