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. 2014 Feb 7;111(8):3092–3097. doi: 10.1073/pnas.1319742111

Fig. 6.

Fig. 6.

Clonality of parathyroid adenomas. (A) Clonality of parathyroid cell populations isolated from five parathyroid adenomas. HUMARA alleles were amplified using methylation-sensitive PCR in HhaI undigested (−) and digested (+) genomic DNA from peripheral blood lymphocytes (PBL), whole tumor tissue (Adenoma), preparatively sorted chief and oxyphil cells, or microdissected tumor tissue (LCM). The presence of a single band reveals a single methylated allele from a monoclonal tumor (Pt3 and -14) whereas two bands reflects random methylation of alleles in a polyclonal tumor (Pt15, -16, and -17). (B) Clonality of 14 parathyroid adenomas identified by HUMARA and PGK methods.