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. 2014 Jan 27;111(8):3068–3073. doi: 10.1073/pnas.1316925111

Fig. 2.

Fig. 2.

Activation of the JAK/STAT pathway by type 1 IFN induces HMGB1 release. (A and B) RAW 267.4 cells were stimulated with IFN-α and IFN-β at various concentrations (A) for the indicated time (B). The levels of HMGB1 in the culture medium were measured by Western blot analysis. (C and D) RAW 267.4 cells were stimulated with IFN-β (100 U/mL) in the absence or the present of pyridone 6 (C) or 2-AP (D) at various concentrations for 16 h. The levels of HMGB1 in the culture medium were measured by Western blot analysis.