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. 2014 Feb 10;111(8):3098–3103. doi: 10.1073/pnas.1308953111

Fig. 5.

Fig. 5.

Transgenic mammary-specific EZH2 overexpression up-regulates NOTCH1 and accelerates tumor initiation in MMTV-neu mice. (A) Whole mounts of mammary glands from 16-wk-old virgin female EZH2+;neu and EZH2wt;neu mice (n = 10 mice per group). (Magnification: 200×.) Average number of tertiary branches ± SD (*P = 0.01). (B) Representative H&E-stained histological sections of mammary glands of EZH2+;neu and EZH2wt;neu mice at 8 and 16 wk of age. Arrow shows atypical intraductal hyperplasia in EZH2+;neu mice. Immunohistochemical detection of EZH2, NICD1, phosphorylated-STAT3 (p-STAT3), and Ki-67 proteins. (Magnification: 400×.) Bar graphs show the percentages of relative expression ± SD at 16 wk quantified using Framework for Image Dataset Analysis (FRIDA) software (*P < 0.0004). (C) Kaplan–Meier curve shows that EZH2+;neu mice (n = 30) formed mammary carcinomas significantly earlier than EZH2wt;neu mice (n = 25) (median time to tumor initiation: 243 d vs. 295 d, respectively; log-rank P < 0.0001). (D) Representative pictures of mammary tumors from EZH2+;neu and EZH2wt;neu mice stained for H&E and immunostained for EZH2 and NICD1. (Magnification: 400×.) Percentage of relative expression ± SD was quantified using FRIDA software (*P < 0.003, **P = 0.009).