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MSCs cultured in the various media can differentiate into chondrocytes. Differentiated cells (A, E, I, R, and N are 10% FBS or 2, 5, 7, or 10% aPRP, respectively) were stained with Hoescht 33342 B, F, K, O, and S are 10% FBS or 2, 5, 7, or 10% aPRP, respectively) and an anti-aggrecan monoclonal antibody (C, G, L, P and T are 10% FBS or 2, 5, 7, or 10% aPRP, respectively). Merged images with brightfield as shown in D, H, M, Q, and U for 10% FBS or 2, 5, 7, or 10% aPRP, respectively.
