Skip to main content
. 2014 Feb 10;14:44. doi: 10.1186/1471-2229-14-44

Figure 1.

Figure 1

Alignment and schematic diagram of EsDREB2B and homolog proteins. A Alignment of the predicted EsDREB2B amino acid sequence with 6 typical DREB2-type proteins from chickpea, soybean, Arabidopsis and rice. Alignment of the predicted polypeptide sequences (residues 1–210) was performed using ClustalX2.1 software. Black and light gray shading indicate identical and conserved amino acid residues. The single lines in black and pink represent predicted motifs (CMIV-1 and CMIV-2) and core negative regulation domain (NRD) of AtDREB2A and GmDREB2A;2 proteins, respectively. The AP2 domain is boxed in black and the three β sheets regions are dash lined in red, the α helix region was labeled in green dash line arrow. Asterisks represent the V14 and E19 conserved amino acids of AP2 domain. The truncated specific CMIV-4 motif was boxed in red. The protein accession numbers are as follows: EsDREB2B (ADV57357.1), CAP2 (ABC49682.1), GmDREB2A;2 (AFU35563.1), GmDREB2B;2 (AAT12423.1), GmDREB2C;2 (AAP83131.1), AtDREB2A (AAL36328.1), OsDREB2B (NP_001055260.2). B Architectures of conserved protein motifs. 17 subtype 1 (DREB2-type) transcriptions factors including E. songoricum, chickpea, soybean, Arabidopsis and rice are collected for motif detection using MEME online tools. Parameters are as follows: 0 or 1 occurrence of a single motif per sequence, motif width ranges of 6 to 50 amino acids, and 10 as the maximum number of motif. CMIV-1(cyan), CMIV-2 (red) and CMIV-3 (green) motifs are reported by Nakano et al. (2006). The AP2 domain (blue) indicate the conserved AP2/ERF DNA-binding domain, the CMIV-4 conserved motif (pink and marked with*) indicate a specific motif shared with truncated DREB2-type proteins. The domains are drawn to scale. AtDREB2A (AAL36328.1), AtDREB2B (AAP37710.1), AtDREB2C (NP_565929.1), AtDREB2E (NP_181368.1), AtDREB2H (NP_181566.2) are from Arabidopsis; GmDREB2A;1 (AFU35562.1), GmDREB2A;2 (AFU35563.1), GmDREB2B;1 (AAQ57226.1), GmDREB2B;2 (AAT12423.1), GmDREB2C;1 (NP_001236509.1), GmDREB2C;2 (AAP83131.1), GmDREB2D;1 (AFU35564.1), GmDREB2D;2 (AFU35565.1) are from soybean; OsDREB2A (NP_001042107.1) and OsDREB2B (NP_001055260.2) are from rice.