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. 2014 Feb 10;14:44. doi: 10.1186/1471-2229-14-44

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Copyright © 2014 Li et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Subcellular localization and transactivation analysis of EsDREB2B protein. A GFP and EsDREB2B: GFP fusion protein were transiently expressed under the control of the CaMV 35S promoter in onion epidermal cells and observed with a laser scanning confocal microscope. The images were presented fluorescence, bright field and merge of bright field and fluorescence. B The transcriptional activation ability of EsDREB2B was examined using the yeast one-hybrid assay. The growth of yeast cells on SD/-His-Trp medium and C β-galactosidase activity was analyzed. PBridge-BD vector served as the negative control and pBridge-BD-GAL4 served as the positive control.