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. 2014 Feb 28;458(Pt 3):449–458. doi: 10.1042/BJ20131520

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© 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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The structure of the hyd operon (STM1539–STM1531). S. enterica was genetically engineered to facilitate isolation of Hyd-5. The strong T5 promoter from the pQE series of expression vectors (Qiagen) was inserted immediately upstream of hydA (STM1539) on the S. enterica chromosome. In addition, a section of the hydA gene encoding a TM was deleted and replaced by the sequence for a His₆ affinity tag. The roles of specific gene products are indicated.