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. 2014 Feb 28;458(Pt 3):449–458. doi: 10.1042/BJ20131520

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© 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) The S. enterica strains LT2a (parent strain), LB03 (P_T5, hydA^ΔTM−His), LB03 H229A and LB03 E73A were grown anaerobically and whole cells were assayed for hydrogen-dependent BV reduction activity. Results are means±S.E.M. (n=3). Inset, anti-Hyd-5 rocket immunoelectrophoresis of periplasmic fractions prepared from the strains LB03 (P_T5, hydA^ΔTM−His), LB03 H229A, LB03 E73A and the parental strain LT2a. The arrow shows the direction of protein migration. (B) SDS/PAGE (12% w/v gel) of pooled IMAC fractions of proteins isolated from strains SFTH06 (P_T5, hydA^His), LB03 (P_T5, hydA^ΔTM−His) (‘ΔTM’), LB03 H229A and LB03 E73A. Images taken from different gels are separated by black borders. Molecular mass is given on the left-hand side in kDa.