Figure 3. 1D slices from the 3D ¹³C-¹³C-¹H NOESY spectrum of wild-type FKBP12.

(A) At the ¹H and ¹³C frequencies for the C^α of Pro⁸⁸ in the minor slow exchange state the NOE cross-peak correlating the sequential C^α of Gly⁸⁹ is more intense than the corresponding NOE cross-peak correlating the intraresidue Pro⁸⁸ C^β interaction. (B) In contrast, the corresponding sequential NOE connectivity correlating the C^α nuclei of Pro⁸⁸ and Gly⁸⁹ in the major slow exchange state is comparatively far less intense than the intraresidue NOE to the Pro⁸⁸ C^β.