Skip to main content
. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Eur J Immunol. 2013 Jul 4;43(9):2327–2337. doi: 10.1002/eji.201243282

Figure 2.

Figure 2

c-Rel is required for T-cell proliferation in response to alloantigen. (A) 2 × 105 purified T cells from c-Rel-/- and WT B6 mice were stimulated with 6 × 105 irradiated TCD-splenocytes from BALB/c mice. [3H]-TdR was added to cell culture 8 hours before the end of culture. Cell proliferation was measured at day 5 by [3H]-TdR incorporation. (B-E) CFSE labeled c-Rel-/- or WT T cells from B6 donors were adoptively transferred into lethally irradiated BALB/c mice. CSFE annexin V profiles and intracellular IFNg expression was measured by flow cytometry on gated live T cells 3 days (B and C) or 4 days (D and E) after BMT. One representative experiment from 3 independent experiments was shown. Panels A, C and E were presented as means ± 1SD of 4 to 5 samples per group. 2 tailed student’s-t test was used for statistical analysis. *P < 0.05; ** P < 0.01; *** P< 0.001.