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Colocalization of DOTAP–DOPC/DNA (panel A), DC-Chol–DOPE/DNA (panel B) lipoplexes and DOTAP–DOPC/P-DNA (panel C), DC-Chol–DOPE/P-DNA (panel D) nanoparticle signals (red) with Lysosensor (lysosome marker, green). (panel E) Fraction of DNA in the lysosomes, F(lys), calculated by using an object-based colocalization protocol.
