Table 1. Data-collection and refinement statistics.

Values in parentheses are for the highest resolution shell.

	Acetate	F6P	S7P	K135M–F6P	K135M–S7P
Data collection
Space group	P212121	P212121	P212121	P212121	P212121
Unit-cell parameters
a (Å)	54.76	56.12	56.32	54.84	54.83
b (Å)	87.02	74.25	74.09	86.73	87.19
c (Å)	140.69	165.00	165.42	140.49	141.14
α = β = γ (°)	90.00	90.00	90.00	90.00	90.00
Resolution range (Å)	29.59–1.80 (1.85–1.80)	28.99–2.00 (2.05–2.00)	28.99–1.65 (1.69–1.65)	29.58–1.80 (1.85–1.80)	29.67–1.50 (1.54–1.50)
Completeness (%)	98.3 (96.4)	99.6 (97.1)	99.9 (99.7)	99.8 (98.6)	100.0 (100.0)
Multiplicity	6.6 (6.5)	4.9 (4.9)	7.3 (7.3)	7.2 (7.2)	7.2 (5.0)
〈I/σ(I)〉	20.2 (4.0)	18.6 (3.6)	29.0 (4.3)	24.5 (3.7)	23.1 (3.7)
R merge † (%)	7.7 (49.1)	8.4 (47.4)	6.8 (53.8)	7.5 (58.2)	6.9 (45.2)
Wilson B factor (Å2)	20.2	21.8	19.8	24.3	17.3
Refinement statistics
Resolution range (Å)	29.59–1.80 (1.85–1.80)	28.99–2.00 (2.05–2.00)	28.99–1.65 (1.69–1.65)	29.58–1.80 (1.85–1.80)	29.67–1.50 (1.54–1.50)
No. of reflections	62125 (4137)	47362 (3101)	84042 (5737)	62778 (4453)	108935 (7982)
R work/R free ‡ (%)	15.1/18.9	18.8/23.2	17.4/20.3	16.0/19.2	14.5/15.9
No. of atoms
Protein	4872	4978	4978	4867	4867
Waters	753	654	979	610	766
F6P/S7P	—	32	36	16	36
Average B factors (Å2)
Protein	23.3	27.2	21.6	27.1	18.5
Waters	32.9	32.2	31.8	37.5	30.4
F6P/S7P	—	16.9	18.2	24.9	16.5
R.m.s. deviations
Bond lengths (Å)	0.020	0.007	0.005	0.011	0.012
Bond angles (°)	1.95	1.19	1.07	1.33	1.52
Ramachandran analysis
Favored regions (%)	95.1	99.3	99.0	99.0	99.0
Allowed regions (%)	100.0	99.7	99.6	99.9	99.7
Disallowed regions (%)	0.0	0.3	0.4	0.1	0.3

^† R _merge = .

^‡ R _work and R _free are defined as R = , where hkl are the reflections used in refinement for R _work and 5% of reflections that were not used in refinement for R _free. F _obs and F _calc are structure factors deduced from measured intensities or calculated from the model, respectively.