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COS-1 cells expressing CFP fusions of Rab29 and Rab38 were left uninfected or were infected with wild-type S. Typhi (wt) or S. Typhi expressing full-length wild-type GtgE (pGtgE) or catalytic triad point mutations of GtgE (pGtgEH151A, pGtgEC45A and pGtgED169A). Two and a half hours after infection, Rab29 and Rab38 were analyzed by Western blotting (a) or fluorescence microscopy (b). Nonspecific bands associated with the Rab38 experiments are indicated by a red asterisk.
