Figure 6.

(a) Full-length GtgE is inhibited by N-ethylmaleimide (NEM), antipain and chymostatin. The reactions with chymostatin and its delivery control, DMSO, were performed separately using identical conditions to those of the reactions with NEM and antipain; thus, this is indicated with a black line. (b) Leupeptin does not inhibit GtgE at the highest concentration that could be tested given the solubility of leupeptin. At a molar ratio of 1:870 GtgE:leupeptin, leupeptin did not inhibit the ability of GtgE to cleave Rab38. The activity of GtgE-Cys45Ala is shown as a control. An asterisk to the right of each panel indicates the row of bands corresponding to GtgE; in (b) a circle denotes the row of bands corresponding to full-length Rab38.