Table 1.

A tabular summary of the NMR structures for γS-WT and γS-G18V structures, calculated using full restraint sets as described in the Methods section. See also Figures S1–S4 and Supplementary Tables 1 and 2.

Restraint Summary	γS-WT	γS-G18V
Total NOE restraints:	7444	4682
intraresidue:	1547	1329
interresidue:
sequential (|i − j| = 1):	1559	972
medium-range (|i − j| ≤ 5):	1286	709
long-range (|i − j| ≥ 6):	3052	1672
Total angular restraints:	408	450
RDCs:	156	147
dihedral (3J coupling):	--	57
dihedral (TALOS+):	252	246
H-bond restraints:	46	90
Structure statistics (20 lowest-energy structures)
Restraint violations
NOE > 0.3 Å	0.6 ± 0.8	0.7 ± 1.1
dihedral > 5°	1.1 ± 1.3	1.6± 0.9
RMSD from ideal covalent geometry
bonds (Å)	0.005 ± 0.000	0.004 ± 0.000
angles (°)	0.582 ± 0.011	0.537 ± 0.016
impropers (°)	0.503 ± 0.013	0.481 ± 0.016
Restraint RMSD
NOE (Å)	0.034 ± 0.025	0.024 ± 0.002
dihedral (°)	0.914 ± 0.137	0.893 ± 0.114
RDC (Hz)	0.238 ± 0.021	0.188 ± 0.018
Average pairwise RMSD (residues 5–178)
backbone RMSD (Å)	0.4	0.5
heavy atom RMSD (Å)	0.8	0.9
RDC Statistics
R-factor (%)	0.642 ± 0.055	0.459 ± 0.046
R-factor (free) (%)	0.817 ± 0.073	0.556 ± 0.052