Figure 6. Depletion of Src kinase by siRNA mimics miR-23b over-expression.
A) Src protein levels were significantly attenuated with 50nM Src-siRNA duplex (Si) compared to a nonsilencing siRNA duplex (Con), (P-PC3, D-Du145). B–C) Cell cycle analysis showing an increase in the G0/G1 phase of PC3 and DU145 cells transfected with siRNA. D–E) Apoptosis assay showing induction of apoptosis after Src knockdown by siRNA in PC3 and Du145 cells. *p<0.05. F) Western blot analysis showing the protein expressions of Src and its downstream genes are rescued by antimiR-23b transfection.