Table 1.

Primer sequences and PCR conditions

	Primer sequence (5′-3′)	Annealing temperature (°C), PCR cycles	Product size (bp)	Reference
Primers for RT-PCR
SFRP5-S	GGCTTGAGCAGCTTCTTCTT	62, 30	395	24
SFRP5-AS	CACTCGGATACGCAGGTCTT
GAPDH-S	CAATGACCCCTTCATTGACC	58, 30	135	8
GAPDH-AS	TGGAAGATGGTGATGGGATT
Primers for sFRP5 bisulfite sequencing
PAN-S	GTTAAGGTAGAGAGATAAGTAAGTG	50, 40	752	We designed
PAN-AS	ACTCCAACAAATTAAACAACC
BSP-S	GGGAGGTAGGGAGTTTTGGGGAGAA	55, 40	277	24
BSP-AS	CCCAAATAAATAACAACCTACRCTAC
Primers for ChIP PCR
SFRP5-S	GAGGCGCCAGGATCAGTC	60, 35	100	We designed
SFRP5-AS	CCGGCCCTGACTCTACCC
GAPDH-S	TACTAGCGGTTTTACGGGCG	60, 35	166	19
GAPDH-AS	TCGAACAGGAGGAGCAGAGAGCGA