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. Author manuscript; available in PMC: 2014 Mar 3.
Published in final edited form as: Mol Cancer Ther. 2011 Nov 4;11(1):244–253. doi: 10.1158/1535-7163.MCT-11-0592

Figure 5. Functional effects of RhoC and FZD4 siRNA knockdown on T24 and J82 cells.

Figure 5

A. mRNA and protein expression level in si-control and si-RhoC transfected bladder cancer cells (T24, J82). WB stands for Western blot analysis. B mRNA and protein expression level in si-control and si-FZD4 transfected bladder cancer cells (T24, J82). At 48 hr after transfection, mRNA and protein were analyzed using real-time PCR and Western analysis. RhoC and FZD4 expression were normalized to β-actin.

C and D. Migration assay of si-RhoC and si-FZD4 transfectants. si-RhoC/si-FZD4 or si-control was transfected into T24 and J82 cells. At 72 hr after transfection, cells were added into the chamber. Cells were incubated for 4 hr at 37°C in a 5% CO2 tissue culture incubator, no migrated cells were removed from transwell membrane filter inserts using cotton-tipped swab and migrated cells were stained with Hema 3 STAIN SET. Representative photomicrographs are shown at 100x magnification.