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. Author manuscript; available in PMC: 2014 Mar 3.
Published in final edited form as: Cancer. 2010 Aug 24;116(24):5637–5649. doi: 10.1002/cncr.25488

Figure 6.

Figure 6

Enrichment of markers for transcriptionally active promoters by miR-205. (A–B) ChIP assay in PC3 cells transfected with miR-205 or cont-miR. Quantification of immunoprecipitated IL24 and IL32 promoter regions were determined by quantitiative real-time PCR and normalized to input DNA. Signals were also confirmed by conventional PCR and PCR product for each sample was resolved on 2% agarose gel and visualized by staining with ethidium bromide. (C) Induction of downstream target genes by miR-205. Since miR-205 induced expression of IL24 and IL32 expression and caused cell cycle arrest and apoptosis in PC3 cells, we examined the expression of several downstream proteins involved in these pathways. Pro-apoptotic and cell cycle checkpoint proteins were induced in response to miR-205 compared to cont-miR.