Figure 1. Development of transgenic tobacco expressing whitefly v-ATPaseA specific dsRNA.

(A) Physical map of dsRNA expression cassette in pBI101. (B) transgenic and control tobacco plants showing comparable morphology. (C) selection of T₁ seeds on kanamycin (300 mg/l) medium, showing non-transgenic seedlings turning white. (D) PCR analysis of transgenic lines in T₁ generation by amplification of nptII gene (upper panel) and amplification of v-ATPaseA+RTM1 intron (lower panel), M; 100 bp DNA ladder; lanes 2–9, different transgenic tobacco lines, lane 10; positive control.