Figure 2. Expression analysis of v-ATPase A specific RNA in different transgenic lines of tobacco.

Dot-blot assay of total RNA from 12 T₁ transgenic lines with probe of (A) v-ATPaseA gene and (B) U6 gene, number shown are different transgenic lines. (C) Dot-blot assay of RNA from control plants (expressing dsRNA of asal gene); RNA was spotted and hybridised with same probes to show specificity of hybridisation. (D) Northern blot analysis of four selected transgenic lines with v-ATPaseA specific probe. Blot showed positive signal from all tested transgenic lines (Lane 2; line 7, Lane 3; line 11, Lane 4; line 16, Lane 5; line 24) while control line (Lane 1; control) did not show any signal. Loading of equal amounts of RNA was confirmed by ethidium bromide staining.