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GENE CLONING
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CaMVE 35S
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GATCTTAATTAACTGCAGGTCCGATTGAGA
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ATGCGGCGCGCCGGATCTTGTAGAGAGAGACTG
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v-ATPaseA sense strand |
GATCGGCGCGCCTTTCCGGACGTTTGGCAGAGA
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ATGCACTAGTGAGAAGTCACCACCAGGAGG
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v-ATPaseA Antisense strand |
GATCGAGCTCTTTCCGGACGTTTGGCAGAGA
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ATGCTACGTAGAGAAGTCACCACCAGGAGG
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RTM intron |
GATCACTAGTGTAAGTCTGATTTTTGACTCTTC
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ATGCTACGTACTGCTGGGTCCAAATCACATA
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nptII
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GAACAAGATGATTGCACGCA
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GAAGAACTCGTCAAGAAGGC
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Asal sense strand |
GATCGGCGCGCCCAGGATGACTGCAAC
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ATGCACTAGTTCCTCCTGAAGCAGCAGGATAT
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Asal Antisense strand |
GATCGAGCTCCAGGATGACTGCAAC
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ATGCTACGTATCCTCCTGAAGCAGCAGGATAT
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RT-PCR
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v-ATPaseA |
ACCACCAGGAGGCCATACC
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CCGGACGTTTGGCAGAGAT
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Actin
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GACCAGCCAAGTCCAAACGA
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CCTTTGTGGTAGAGGTCTCAGTT
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