Effects of MnTMPyP, rotenone, and cytochalasin B on stress-induced DHE staining. Sequestering superoxide produced significant reductions in DHE staining at both 0.5MPa and 0.25MPa (p<0.05). Inhibition of mitochondrial electron transport with rotenone decreased DHE staining after 1 hour of 0.25MPa static compressive stress (p<0.05). Cytoskeletal dissolution with cytochalasin B significantly decreased DHE staining at 0.25MPa (p<0.05). Statistical significance markers (p<0.05) are denoted as α indicating significance vs. 0.25MPa untreated specimens, β vs. 0.5MPa untreated specimens, γ vs. 0.5MPa rotenone treatment, and δ vs. 0.5MPa cytochalasin B treated.