Figure 1. Dependency of specific iodide oxidation rates on iodide concentration for T. versicolor laccase.

Reaction conditions: Citrate-phosphate buffer pH 5.0, room temperature, 0.01 or 0.1 mg mL⁻¹ enzyme. Closed triangles: without organic substrate, open triangles: with vanillin (100 µM), filled circles: with mediator ABTS (10 µM), open circles: with mediator acetosyringone (10 µM). Average values and standard deviations of n = 3 replicate assays per concentration. Black lines: best fit (least squares) of Michaelis-Menten models (incl. substrate inhibition).