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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: J Pathol. 2013 Mar 14;230(1):82–94. doi: 10.1002/path.4171

Figure 5. Downregulation of miR-26a and upregulation of iNOS protein expression in NPM- ALK+ T-cell lymphoma is mediated through an NPM-ALK/STAT3 signaling axis.

Figure 5

Figure 5

(A) Western blot shows that downregulation of ALK by siRNA in Karpas 299 and DEL cell lines was associated with a marked decrease in pSTAT3 and iNOS proteins; (B) Transfection of STAT3 siRNA decreased iNOS protein levels; (C) qPCR analysis shows that transfection of Karpas 299 and DEL cell lines with ALK or STAT3 siRNA increased miR-26a levels (*: p < 0.05, **: p < 0.00001); (D) ALK and STAT3 siRNA did not cause significant changes in iNOS mRNA levels by qPCR analysis. In all experiments, control cells were transfected with scrambled siRNA. Experiments were repeated 3 times and the data represent means ± SE.