Figure 5. Embryos derived from transgenic line huORFZ provide true signals of a contaminated aquatic environment.

The GFP fluorescent signal intensities induced in huORFZ embryos showed responses relative to different river samples collected from local waterways. In Sample 1, no GFP signal was observed in huORFZ embryos, consistent with WHO water safety standards. In Sample 2, the GFP response in skin tissue of huORFZ embryos indicated potential copper pollution. In Sample 3, GFP signals in the brain of huORFZ embryos corresponded to embryonic toxicity consistent with high Zn²⁺ levels. Finally, in Sample 4, the GFP response shown in CNS of huORFZ embryos was attributed to the presence of multiple pollutants, such as Zn²⁺ and Pb²⁺. All images are lateral views with anterior to the left.