Table 3. List of Primers.

Name	Sequence 5′->3′	Start Position on Scaffold 5 (nt)	Len	Notes
Seq16F	ATTCCGCTCCTCATGTAGTACA	15,898,340	22	upstream the LTR insertion
Seq4R	CCAAATAAACCACCACCTACTCTGTTA	15,899,297	27	downstream the LTR insertion
Seq15F	GCTGTAGGCTAAGGTGGAGAA	15,898,078	21	RT-PCR primer designed on exon 2 of PpeMYB25
Seq15R	ACTGAGCAATGTGGCTGAGA	15,898,586	20	RT-PCR primer designed on exon 3 of PpeMYB25
ppaRPII_RT-F	TGAAGCATACACCTATGATGATGAAG		26	RT-PCR housekeeping, [53](Tong et al, 2009)
ppaRPII_RT-R	CTTTGACAGCACCAGTAGATTCC		23	RT-PCR housekeeping, [53](Tong et al, 2009)
indelG-F	CTTGCACCTGAGTTCGATTCCG	15,898,324	22	upstream the indel in PpeMYB25
indelG-1R	GGCTTCAATGGCAGAACAAGG		21	within left border of the indel in PpeMYB25
indelG-2R	GCAGGTGGTGGAGATTCATTCAT	15,899,264	23	downstream the indel in PpeMYB25

Name, sequence, position on Peach v1.0 and length of primers used to perform long-range PCR, RT-PCR and indelG assay.