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. 2014 Mar 3;9(3):e90590. doi: 10.1371/journal.pone.0090590

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© 2014 Whitesell et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Confocal images of 4 and 7(acta2:mCherry) and nuclear neural crest marker (fli1a:nEGFP^y7) using ventrally staged embryos. (A) Mural cell and neural crest markers are expressed along the ventral aorta (A’) and aortic arch artery region (A”) of the 4 dpf embryo. (B) Mural cell and neural crest markers are expressed along the ventral aorta (B’) and aortic arch region (B”) at 7 dpf. There appears to be little to no co-localization of fluorescent markers at both 4 and 7 dpf. Scale bar in A represents 100 µm. Insets (A’, A”, B’, B”) are 100 µm in length. VA = Ventral Aorta, AAA = Aortic Arch Arteries. Arrowheads depict cells that no do not co-localize.