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. 2014 Mar 3;9(3):e90803. doi: 10.1371/journal.pone.0090803

Figure 2. Very low eIF2α-P levels in striatal cells, much increased by expression of Htt111Q.

Figure 2

A) Basal level of eIF2α-P in murine cell lines normalized by total eIF2α. Graph: average of 3 experiments ± SE. **P = 0.004, ***P  = 0.001. B) Immunofluorescence images of cells fixed, permeabilized and stained with rabbit anti-eIF2α-P and mouse anti-eIF2α followed by secondary antibodies. Bar = 10 µm. Image exposure time was kept constant to be able to compare protein levels in the different cell types. Levels relative to STHdh Q7/7 levels were quantified from images from 3 experiments ± SE (>20 cells, ***P<0.001).