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. 2014 Mar 3;9(3):e90803. doi: 10.1371/journal.pone.0090803

Figure 5. High sensitivity of striatal neurons to ER stress, further aggravated by expression of pathogenic huntingtin.

Figure 5

A-C) Strong induction of GADD34 and CHOP upon prolonged ER stress in STHdh Q7/7 cells and even stronger in STHdh Q111/111 cells; (3 independent experiments ±SE). *P = 0.02, **P = 0.01, ***P = 0.0002. Immunoblots of a representative experiment are shown in A. GAPDH levels served here as a loading control. D) Prolonged ER stress induced with Tun or MG-132 leads to extensive death of STHdh Q7/7 cells, further aggravated in STHdh Q111/111 cells, as measured by FACS analysis of cell cycle progression with propidium iodide (PI) (see Fig. S2); (6 independent experiments ± SE). *P<0.05, **P = 0.01, ***P = 0.001.