Figure 6.
NDRG2 knockdown promotes TGF-β-induced EMT. (a) Phase-contrast photomicrographs of control cells and cells treated with TGF-β demonstrated that NDRG2 and p21Cip1 knockdown mimics the EMT phenotype in HT29 cells. (b) Circularity index of untreated and TGF-β1-treated cells. (c) Western blot analysis of E-cadherin and vimentin expression in total lysates from untreated cells and cells treated with 10 ng/ml TGF-β for 48 h. (d, e) Migratory and invasive behavior of untreated cells and cells treated with 10 ng/ml TGF-β for 48 h. The control values were normalized to 1, and the data are expressed as the fold-change in treated cells. (f) The expression level of mRNAs encoding E-cadherin, N-cadherin, fibronectin, Twist, Snail and vimentin in control cells and cells treated with 10 ng/ml TGF-β for 48 h. (d–f) The data are presented as the means±s.d. from triplicate analysis.