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. 2014 Feb 3;3(2):e85. doi: 10.1038/oncsis.2013.49

Figure 1.

Figure 1

DNA-PKcs is required for Chk2 Thr68 phosphorylation during mitosis. (a) Human colon cancer HCT116 cells and derivative DNA-PKcs−/− cells were synchronized with nocodazole (50 ng/ml, 16 h) or were irradiated (4 Gy, 1 h). Whole-cell lysates were separated by electrophoresis and western blotted with the indicated antibodies. (b) HeLa cells transfected with a control siRNA or with an siRNA against DNA-PKcs were synchronized with nocodazole (50 ng/ml, 16 h) or were irradiated (4 Gy, 1 h). Whole-cell lysates were separated by electrophoresis and were western blotted with the indicated antibodies. Asynchronous (Asy), mitosis enriched (M), γ-ray irradiation (IR).