Figure 1. Viability and ROS-level of γ-synuclein ab treated and stressed RGC-5.

RGC-5 were preincubated with different γ-synuclein ab concentrations and additionally stressed with 20 mM glutamate for 24 h and 50 µM H₂O₂ for 1 h. Cell viability as well as ROS-level were determined using crystal violet and DCFH-DA (* = p<0.05; **p<0.01) A: Increased significant and high significant cell viability were measured after the cells were preincubated with 0.05–5 µg/ml γ-synuclein abs and additionally stressed with H₂O₂. B: Significant and high significant decreased ROS production was measured, when the cells were preincubated with 0.1 and 5 µg/ml γ-synuclein abs. C: Increased cell viability in a range of 0.05–5 µg/ml γ-synuclein abs were obtained after the cells were stressed with glutamate, whereby the results of 0.1 and 1 µg/ml are high significant.