Figure 5.

Wheel-running activity alters the eating behavior rhythm in mice fed high-fat diet. Male wild-type mice were single-housed in 12L: 12D with a locked (A,B) or freely rotating (C,D) running wheels at 7 weeks old. Chow was provided ad libitum for 1 week (Days 1–7) and then chow was replaced with high-fat diet (HFD) for 1 week (Days 8–15). Representative circular histograms (A–D; plotted in 10-min bins; scale: inner circle, 0; middle circle, 5.5, outer circle, 11; units: eating events per 10-min bin) show the distribution of eating events for a mouse during chow (Day 7; left panels) and HFD (Day 9; right panels) consumption relative to the time of day (where 0 is lights on and 12 is lights off). Grand mean vectors of eating behavior (E; n = 5 mice/group) during chow (black and green arrows) or HFD (blue and red arrows) feeding in mice with locked (black and blue arrows) or freely-rotating wheels are plotted on the circular histogram (plotted in 10-min bins; scale: inner circle, 0.3; outer circle, 0.6). The size and length of the arrow represents the uniformity of the distribution of the eating events where small, short arrows indicate that eating events are more evenly distributed across the cycle.