Table 1.
Circadian parameters of bioluminescence rhythms in ex vivo tissues.
| Locked wheel | Free wheel | |||||
|---|---|---|---|---|---|---|
| Tissue | Chow: mean ± SD (n) | HFD: mean ± SD (n) | Chow: mean ± SD (n) | HFD: | P* | |
| Period (h) | SCN | 24.34±0.25 (7) | 24.35±0.11 (7) | 24.29±0.24 (6) | 24.23±0.19 (6) | NS |
| Pituitary | 23.37±0.27 (6) | 23.73±0.59 (7) | 23.47±0.47 (6) | 23.46±0.50 (6) | NS | |
| Liver | 21.59±0.66 (5) | 21.28 h±0.60 (5) | 20.68±1.03 (5) | 20.24±0.54 (6) | Wheel: F(1, 17) = 9.52 p < 0.01 | |
| Lung | 23.98±0.35 (6) | 23.99±0.45 (7) | 24.00±0.40 (6) | 23.76±0.18 (6) | NS | |
| Spleen | 24.40±0.44 (6) | 24.34±0.33 (7) | 24.42±0.83 (6) | 24.15±0.34 (6) | NS | |
| Aorta | 24.54±0.17 (4) | 24.53±0.33 (3) | 24.03±0.36 (6) | 23.91±0.49 (6) | Wheel: F(1, 15) = 9.66 p < 0.01 | |
| Arcuate complex | 23.37±0.88 (3) | 23.17±0.51 (5) | 22.53±0.36 (4) | 22.35±0.67 (4) | Wheel: F(1, 12) = 7.40 p = 0.01 | |
| Gonadal WAT | 25.85±0.54 (4) | 25.59±0.77 (6) | 25.62±0.51 (6) | 25.74±0.43 (6) | NS | |
| Amplitude of bioluminescence (counts/s) | SCN | 36.03±14.35 (7) | 27.61±11.79 (7) | 48.89±12.90 (6) | 52.17±33.84 (6) | Wheel: F(1, 22) = 5.77 p = 0.03 |
| Pituitary | 37.61±11.16 (7) | 29.15±11.23 (6) | 54.58±26.04 (6) | 37.91±10.24 (6) | NS | |
| Liver | 9.43±5.38 (6) | 18.32±13.82 (6) | 8.97±4.29 (5) | 10.96±10.62 (6) | NS | |
| Lung | 26.71±8.31 (7) | 21.89±8.68 (7) | 34.47±12.34 (6) | 22.15±8.69 (6) | Diet: F(1, 22) = 5.21 p = 0.03 | |
| Spleen | 23.58±9.98 (7) | 21.57±6.23 (7) | 30.14±12.92 (6) | 27.24±5.85 (6) | NS | |
| Aorta | 25.61±13.18 (3) | 15.70±2.59 (3) | 38.32±15.74 (5) | 34.15±19.65 (6) | NS | |
| Arcuate complex | ND | ND | ND | ND | ND | |
| Gonadal WAT | 11.27±6.3 (6) | 15.81±7.03 (3) | 15.04±6.33 (6) | 9.13±9.39 (6) | NS | |
*
The data were compared by 2 × 2 factorial analysis. None of the tissues had a significant interaction between wheel and diet. Some tissues showed a significant effect of wheel or diet, as reported in the table. NS indicates that no significant differences were found.