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. 2014 Jan 17;5:3075. doi: 10.1038/ncomms4075

Figure 5. Characterization of the AAV2R585E mutants carrying amino acids responsible for galactose binding.

Figure 5

(a) Capsid amino-acid sequences of a series of the AAV2R585E-derived mutants. (b) Cell surface binding of the AAV2R585E mutants. We produced dsAAV-CMV-GFP vectors packaged in each AAV2R585E mutant capsid. We exposed Pro5 or Lec2 cells to these mutant vectors at an MOI of 105, at 4 °C for 1 h, and determined the quantity of cell surface-bound AAV particles by qPCR of the viral genome (n=3). (c,d) We applied the AAV2R585E mutant GFP vectors to Pro5 or Lec2 cells at an MOI of 106, and determined transduction efficiency 48 h after infection by fluorescent microscopy (c) and flow cytometry (d) (n=3). In the bar graphs, error bars represent s.e.m. This experiment was performed once. Scale bar, 400 μm.