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. 2014 Jan 6;5:2958. doi: 10.1038/ncomms3958

Figure 7. ROS directly affect the intermolecular disulphide bond between TRX and TXNIP.

Figure 7

(a,b) Size-exclusion chromatography analyses of the effect of ROS on the interaction between TRX and TXNIP. The TRX-T–TXNIP(C120S/C170S/C205S/C267S) complex was incubated with 0, 5, or 10 mM H2O2 at 37 °C for 30 min (a) and the TRX(C73A)–T–TXNIP(C36S/C49S/C120S/C170S/C205S/C267S) complex was incubated with 0 or 3 mM diamide at 37 °C for 30 min (b). The reaction products were injected onto a HiLoad 16/60 Superdex 75 gel filtration column at room temperature. (c) The TRX(C35A)–T–TXNIP(C120S/C170S/C205S/C267S) complex was incubated with 3.3 mM diamide at 37 °C for 60 min. The reaction products were injected into a Superdex 75 10/300 GL gel filtration column at room temperature. (a–c) All eluted proteins were analysed by SDS–PAGE. T–TXNIP(m), T–TXNIP mutant; LS, loading solution.