Skip to main content
. 2014 Mar 5;3:e01440. doi: 10.7554/eLife.01440

Figure 1. Summary of Nasonia eve mRNA expression.

Embryos are shown with anterior left and dorsal up. Nv eve is initially expressed in a broad domain (A and B), which sharpens as a posterior stripe becomes visible at around 4 hr after embryo laying (AEL) (C and D). The broad domain retracts anteriorly and gives rise to three apparently double-segment stripes (E and F). Between stripes 3 and posterior stripe 6, an additional double stripe precursor comes up at around 6 hr AEL (stripe 4/5; panels F and G) and this splits to form two double-segment stripes, ‘4’ and ‘5’ as double-segment stripes 1–3 split into two single-segment stripes each between 6 and 8 hr AEL (F–J). Stripes 4 and 5 also split to form single-segment stripes during early gastrulation, and stripe 6 broadens (K and L), giving rise to stripes that are visibly distinct during germ band extension in non-fluorescent staining by 10–12 hr AEL (M–R, arrowheads). There are a total of 16 single-segment stripes of Nv eve.

DOI: http://dx.doi.org/10.7554/eLife.01440.003

Figure 1.

Figure 1—figure supplement 1. Nv wingless (Nv wg) mRNA expression in the embryo.

Figure 1—figure supplement 1.

All embryos are shown with anterior to the left and dorsal up except as indicated. (A) Precellular blasoderm with no expression of Nv wg. (B) Cellular blastoderm embryo exhibiting head expression and one stripe of Nv wg. (C) Early gastrula embryo exhibiting Nv wg staining in three segmental stripes comprising thoracic segments. (D) Ventral view of early gastrula embryo with four segmental stripes, including one abdominal segment. (E) Germ band extension embryo with six segmental stripes of Nv wg expression. (F) Germ band extension embryo exhibiting eight segmental stripes, including three thoracic and five abdominal stripes, as well as additional head segmental stripes. (G) Ventral view of a fully extended germ band with the full complement of 16 segmental Nv wg stripes. (H) Lateral view of germ band retracting embryo with 16 segmental stripes, including three clear segmental stripes in the head. (I) Lateral view of dorsal closure embryo exhibiting fading segmental Nv wg staining in all segments.
DOI: http://dx.doi.org/10.7554/eLife.01440.004