Figure 6. Phasing of Nasonia pair-rule genes in embryos using double fluorescent in situ hybridization.

(A) Lateral view of Nv eve expression in early gastrula embryo. (B) Nv odd expression alone in the same embryo. (C) Merge of Nv eve and Nv odd channels, illustrating their relative phasing. Nv eve mRNA is pseudo-colored pink, Nv odd is in green. Arrowheads indicate position of a posterior doublet of odd stripes. (D) Dorsolateral view of Nv eve in later gastrula embryo. (E) Nv odd expression alone in the same embryo. Arrowheads indicate position of posterior Nv odd stripes 6, 7 and 8. (F) Merge of Nv eve and Nv odd channels, illustrating their relative phasing. (G) Lateral view of Nv eve expression in blastoderm embryo. Arrowhead indicates position of Nv eve stripe 5. (H) Nv runt expression in the same blastoderm embryo. (I) Merge of Nv eve (green) and Nv runt (pink) channels, indicating relative phasing. (J) Lateral view of Nv eve expression in germ-band-extended embryo. Numbers indicate identity of Nv eve stripe. (K) Nv runt expression alone in the same embryo. Arrowheads indicate position of posterior primary Nv runt stripes. (L) Merge of Nv eve (green) and Nv runt (pink) channels, indicating relative phasing. Note that posterior Nv runt stripes, though faint, appear to be positioned posterior to odd-numbered Nv eve segmental stripes. (M) Lateral view of Nv eve expression in early gastrula embryo. Line indicates broadening stripe 6. (N) Nv hairy expression in the same gastrula embryo. Arrowheads indicate positions of three late forming posterior double-segment stripes. (O) Merge of Nv eve (pink) and Nv hairy (green) channels, indicating relative phasing. (P) Ventral view of gastrula embryo showing Nv eve expression alone. Arrowheads indicate positions of single-segment stripes derived from eve stripe 6. (Q) Nv hairy expression alone in the same gastrula embryo. Line indicates extended anterior domain continuous with stripe 1. (R) Merge of Nv eve (green) and Nv hairy (pink) channels, illustrating relative phasing.

DOI: http://dx.doi.org/10.7554/eLife.01440.014

Figure 6—figure supplement 1. Summary of Nv runt mRNA expression.

Embryos are shown anterior left and ventral up unless indicated. (A) Early energid stage embryo with faint staining evident in posterior. (B) Early precellular blastoderm embryo exhibiting stronger, mostly ubiquitous Nv runt staining biased towards the posterior. (C) Precellular blastoderm embryo with strong stripe of Nv runt expression within larger, gap-type domain. Expression is absent from the rest of the embryo. (D) Precellular blastoderm embryo exhibiting three clear pair-rule stripes of Nv runt expression. (E) Cellular blastoderm embryo with four pair-rule stripes of Nv runt expression. (F) Early gastrula embryo exhibiting three pair-rule stripes, an expanding stripe 4/5 stripe domain and faint posterior stripe 6. (G) Early gastrula embryo exhibiting 6 pair-rule stripes of Nv runt expression. (H) Gastrula embryo exhibiting splitting of anterior stripes 1 and 2 (and possibly 3), and stronger expression of stripes 4 and 5, with expanded interstripe distance between stripes 5 and 6. (I) Dorsal view of gastrula embryo at approximately the same stage as in (H). (J) Dorsolateral view of germ-band extension embryo with fading anterior stripes, and an additional pair-rule stripe appearing between stripes 5 and 6 (arrowhead). (K) Germ-band extension embryo exhibiting seven pair-rule stripes of expression with segmental stripes appearing in posterior interstripes (arrowheads). (L) Ventral view of fully extended germ band exhibiting segmental expression in all segments, in addition to continuous expression in the ventral anterior of the embryo.

DOI: http://dx.doi.org/10.7554/eLife.01440.015