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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Eur J Immunol. 2013 Oct 20;44(1):239–250. doi: 10.1002/eji.201343774

Figure 1. Coexpression of human CD8 and gp209-specific TCRs on hybridomas improves T-cell function.

Figure 1

(A) Six gp209-specific TCRs isolated from melanoma patients listed in order of descending 3D affinities. (B) The TCRs and human CD8 were expressed on hybridomas at similar surface densities as quantified by flow cytometry. (C) Cytokine release of hybridomas expressing TCR and CD8 stimulated with T2 cells loaded with various amounts of gp209-2M peptide. Data are shown as mean ± STD (standard deviation) of triplicate measurements.