Figure 2. Correlation of 3D parameters to T-cell function.

(A) Correlation between 3D affinity determined by SPR and quantification of IL-2. (B) Tetramer staining MFI of CD8⁺ or CD8⁻ hybridomas expressing the indicated TCRs. (C) Correlation between tetramer staining and 3D affinity. (D) Correlation between tetramer staining and IL-2 secretion. The solid lines represent linear regression with R² and p values indicated. In panels A&D, only IL-2 secretion at a representative peptide concentration (8 μM) is shown; comparable results were obtained when using different peptide concentrations (see Materials and methods and Supporting Information Table 1).