Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Mar 3;204(5):759–775. doi: 10.1083/jcb.201308026

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Cijsouw et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — Synaptic activity increases Munc18-1-Venus mobility and recruits Munc18-1 to synapses independent of syntaxin-1. (A) Fluorescence and greyscale images of Munc18-1-Venus (M18V) axons expressing synapsin-mCherry. Greyscale images show the same axon before (pre), during (t = 12 s), and after stimulation (t = 198 s, M18V only) with 600 AP at 20 Hz (start t = 0 s). Bar, 5 µm. (B) Relative M18V intensity changes (ΔF/F0) in synapses marked by arrowheads in A. Black bars indicate the period of stimulation. Some synapses, after initial dispersion during stimulation, increase fluorescence above initial levels (broken line), whereas others remain low after initial dispersion or do not change during measurement. (C) Relative M18V intensity changes of all synapses (green line, n = 23 field of views, n = 510 synapses) compared with subsets (gray lines) of synapses with net positive change (Δ+, 46.3% of all synapses) and synapses with net negative change (Δ−, 37.8%) at t = 160 s. (D) Relative Stx-YFP intensity changes of all synapses (blue line, n = 10 field of views, n = 125 synapses) and of subsets (gray line) of synapses with net positive change (Δ+, 22.4% of all synapses) or a net negative change (Δ−, 36.0%) at t = 160 s. (E) Relative M18V intensity changes of all synapses (green, n = 5 cells, n = 553 synapses) and M18V ± BoNT/C synapses (yellow, n = 5 cells, n = 290 synapses). (inset) Percentage of synapses with net positive change (−BoNT/C, 38.6%; +BoNT/C, 44.1%) and synapses with net negative change (−BoNT/C, 31.9%; +BoNT/C, 19.3%) at t =160 s (Pearson χ² test: ***, P < 0.001). (F) Relative M18V intensity change at t = 10 s (left) and t = 160 s (right) at control synapses (green) and synapses expressing BoNT/C (yellow) calculated from E (M-W test with FDR [2] corrections: ***, P < 0.001). (G) Relative M18V and synapsin-mCherry (inset) intensity changes of control synapses (green, n = 6 cells, n = 250 synapses) and synapses superfused with calcium channel blockers to prevent calcium influx (brown, n = 6 cells, n = 252 synapses). Synapsin-mCherry intensity at t = 30 s, control versus calcium channel blockers, M-W test: ***, P < 0.001). (H) Relative M18V intensity change at t = 10 s (left) and t = 160 s (right) at control synapses (green) and synapses superfused with calcium channel blockers (brown) calculated from G (M-W test with FDR [2] corrections: n.s., P > 0.05; ***, P < 0.001). Note that although average dispersion (t = 10 s) in control synapses is lower compared with E, Fig. S5 shows that dispersion in Δ+ and Δ− control synapses is significantly higher than in synapses with calcium channel blockers. The horizontal broken line indicates no change.