Figure 2.

Mapping the interaction domain of Arp5 with Myocd. (A) Schematic representation of the domain deletion constructs of Arp5. Numbers indicate amino acid positions. S1, S2, and S3 are N-terminal, central, and C-terminal sequences, respectively, unique to Arp5. N- and C-domain are conserved N- and C-terminal regions, respectively, between Arp5 and β-actin. The similarities between Arp5 N/C-domains and β-actin N/C-domains are indicated as percentages. (B) The indicated expression vectors were transfected into HeLa cells with the Myocd expression vector and 3× CArG-Luc, and the luciferase reporter assay was then performed. Data represent the mean ± SEM of four independent experiments. *, P < 0.05, Student’s t test. (C–F) Hek293T cells were transfected with the indicated combinations of expression vectors and coimmunoprecipitation was performed.