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. 2014 Mar 3;204(5):839–856. doi: 10.1083/jcb.201306088

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© 2014 Shamir et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — Organoids recovered epithelial behaviors when Twist1 was turned off. (A) Twist1 was transiently activated in CMV::rtTA;TRE-Twist1 epithelium by a 48-h pulse of doxycycline. (B) In basal medium, organoids transiently disseminated, but disseminated cells stopped migrating after doxycycline removal (arrowheads; Video 6). (C–E) In the presence of FGF2, organoids initiated new buds after doxycycline removal (D), and disseminated cells reintegrated with the main organoid (E; Video 7). (F) Branched organoids displayed normal mammary epithelial organization, with inner luminal epithelial cells (K8⁺) and outer myoepithelial cells (K14⁺). Bars, 20 µm.