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. 2014 Feb 7;8(Suppl-1):27–35. doi: 10.2174/2213988501408010027

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© Reid et al.; Licensee Bentham Open.

This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

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Fig. (4) — A robust algorithm can capture both local (single plane) data and global (whole spheroid) phenomena. Using a specific field within a well of a 96-well plate an algorithm for analysis of CK5Pro-GFP expression in MCTS can be used to capture data in a single plane or globally by using maximal projections. Panels 1-4 (bottom right) show the maximal projection of 8 Z-planes. Panel 1 shows the stained nuclei (Hoechst, blue) and the CK5Pro-GFP expression (green). Panel 2 shows the regions of the image tiled with spots (arbitrary colors). Panel 3 shows spots selected by fluorescence intensity and size (selected spots in green, rejected spots in red). Panel 4 shows the entire spheroid region across which high-content parameters were summed.